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培養基配方

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Clostridium aminobutyricum Medium

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓普(pu)生物工程有限公司(si) 培養基(ji)配方 //ljxinglong.com

Composition per liter:

K2HPO4.............................................................7.05g

Yeast extract........................................................3.0g

KH2PO4.............................................................1.29g

MgCl2·6H2O .......................................................0.2g

CaCl2·2H2O ......................................................0.01g

FeCl3·6H2O.......................................................0.01g

Methylene Blue................................................2.0mg

MnSO4·H2O.....................................................1.0mg

Na2MoO4·2H2O ...............................................1.0mg

γ-Aminobutyrate solution ........................... 100.0mL

Na2CO3 solution ......................................... 100.0mL

Na2S·9H2O solution...................................... 50.0mL

pH 7.4–7.7 at 25°C

γ-Aminobutyrate Solution:

Composition per 100.0mL:

γ-Aminobutyrate .................................................5.0g

Preparation of γ-Aminobutyrate Solution:

Add γ-aminobutyrate to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Na2CO3 Solution:

Composition per 100.0mL:

Na2CO3 ...............................................................2.0g

Preparation of Na2CO3 Solution: Add Na2CO3

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Na2S·9H2O Solution:

Composition per 50.0mL:

Na2S·9H2O..........................................................0.3g

Preparation of Na2S·9H2O Solution: Add

Na2S·9H2O to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Add components, ex

cept γ-aminobutyrate solution, Na2CO3 solution, and

Na2S·9H2O solution, to distilled/deionized water and

bring volume to 750.0mL. Autoclave for 15 min at 15

psi pressure–121°C. Cool under 80% N2 + 10% CO2

+ 10% H2. Aseptically add the sterile γ-aminobu

tyrate solution, Na2CO3 solution, and Na2S·9H2O so

lution. Adjust pH to 7.4–7.7. Distribute using

anaerobic technique into tubes or flasks.

Use: For the cultivation and maintenance of Clostrid

ium aminobutyricum and other bacteria which can uti

lize aminobutyric acid as a carbon source.

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Clostridium aminobutyricum Medium