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培養基配方

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Christensen’s Urea Agar (BAM M40)

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]

Christensen’s Urea Agar

(BAM M40)

山東拓普(pu)生(sheng)物工程有限(xian)公司培養(yang)基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

NaCl....................................................................5.0g

KH2PO4...............................................................2.0g

Peptone ...............................................................1.0g

Glucose ...............................................................1.0g

Phenol Red......................................................0.012g

Urea solution............................................... 100.0mL

pH 6.8 ± 0.1 at 25°C

Urea Solution:

Composition per 100.0mL:

Urea...................................................................20.0g

Preparation of Urea Solution: Add urea to

100.0mL of distilled/deionized water. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, ex

cept urea solution, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50–55°C. Aseptically

add 100.0mL of sterile urea solution. Mix thorough

ly. Pour into Petri dishes or distribute into sterile

tubes. Allow tubes to solidify in a slanted position.

Use: For the differentiation of a variety of microor

ganisms, especially members of the Enterobacteri

aceae, aerobic actinomycetes, streptococci, and

nonfermenting Gram-negative bacteria, on the basis

of urease production.

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