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Christensen Citrate Sulfide Medium

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Christensen Citrate Sulfide Medium

山東拓普生物工程有限公(gong)司 培養基配(pei)方(fang) //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
NaCl....................................................................5.0g
Sodium citrate·2H2O...........................................3.0g
KH2HPO4............................................................1.0g
Yeast extract........................................................0.5g
Ferric citrate........................................................0.2g
Ammonium citrate ..............................................0.2g
Glucose ...............................................................0.2g
L-Cysteine·HCl·H2O ...........................................0.1g
Na2S2O3·5H2O ..................................................0.08g
Phenol Red......................................................0.012g
pH 6.7± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Dispense
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave
in tubes. Allow tubes to cool in a slanted position.
Use: For the differentiation of enteric pathogens, es
pecially members of the Enterobacteriaceae, and
coliforms based on their ability to utilize citrate as a
carbon source and production of H2S. Bacteria that can
utilize citrate turn the medium pink-red. H2S produc
tion appears as a blackening of the butt of the tube.
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