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Chopped Meat Carbohydrate Medium

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Chopped Meat

Carbohydrate Medium

山(shan)東拓普生(sheng)物(wu)工程有限公司 培養(yang)基配方 //ljxinglong.com

Composition per 1240.0mL:

Peptone..............................................................30.0g
K2HPO4...............................................................5.0g
Yeast extract........................................................5.0g
Cellobiose ...........................................................1.0g
Maltose................................................................1.0g
Starch ..................................................................1.0g
L-Cysteine·HCl·H2O............................................0.5g
Chopped meat extract filtrate............................. 1.0L
Chopped meat extract solids....................... 200.0mL
Resazurin (0.025% solution)........................... 4.0mL
pH 7.0 ± 0.2 at 25°C
Chopped Meat Extract:
Composition per liter:
Beef or horse meat ..........................................500.0g
NaOH (1N solution)...................................... 25.0mL
Preparation of Chopped Meat Extract: Use
lean beef or horse meat. Remove fat and connective
tissue. Grind. Add meat and NaOH to distilled/deion
ized water and bring volume to 1.0L. Gently heat and
bring to boiling while stirring. Cool to 25°C. Remove
fat from surface. Filter. Reserve ground meat parti
cles and filtrate. Add distilled/deionized water to fil
trate and bring volume to 1.0L.
Preparation of Medium: To 1.0L of chopped
meat extract filtrate, add the remaining components,
except the L-cysteine·HCl·H2O and chopped meat
solids. Mix thoroughly. Gently heat to boiling. Cool
to room temperature. Add the L-cysteine·HCl·H2O.
Adjust pH to 7.0. Distribute 1 part chopped meat sol
ids (by volume) and 5 parts of liquid (by volume) into
tubes under O2-free 97% N2 + 3% H2. Cap with rub
ber stoppers and place tubes in a press. Autoclave for
15 min at 15 psi pressure–121°C with fast exhaust.
Use: For the cultivation of anaerobic bacteria, in
cluding Clostridium species.
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