99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開
個人中心 我的訂單 購物車
編號：
批號：
培養基配方
您現在的位置： 網站首頁 >> 培養基配方

Chopped Meat Broth

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Chopped Meat Broth

山東拓普生物工(gong)程(cheng)有限公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Ground meat, fat free......................................500.0g
Pancreatic digest of casein................................30.0g
K2HPO4...............................................................5.0g
Yeast extract........................................................5.0g
L-Cysteine·HCl ...................................................0.5g
Resazurin .........................................................1.0mg
NaOH (1N solution)...................................... 25.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Use lean beef or horse
meat. Remove fat and connective tissue. Grind fine
ly. Add ground meat and 25.0mL of NaOH solution
to distilled/deionized water and bring volume to
1025mL. Gently heat and bring to boiling. Continue
boiling for 15 min without stirring. Cool to room
temperature. Remove fat from surface. Filter and re
tain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add
pancreatic digest of casein, K2HPO4, yeast extract,
and resazurin. Gently heat and bring to boiling. Boil
for 1–2 min. Add L-cysteine·HCl. Mix thoroughly.
Distribute 7.0mL into tubes that contain meat parti
cles (1 part meat particles to 5 parts fluid). Autoclave
for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of various anaerobes.
  版權所有：山東拓普生物工程有限公司 營銷服務熱線：　郵箱：
© 2016-2020 ALL RIGHTS RESERVED.　　
購物車 去頂部