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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Andrade’s Broth
Composition per liter:
Pancreatic digest of gelatin.............................................10.0g
NaCl......................................................................5.0g
Beef extract..............................................................3.0g
Andrade’s indicator......................................................10.0mL
Carbohydrate solution......................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems, in tubes containing adonitol, arabinose, cellobiose,
dulcitol, fructose, galactose, glucose, inositol, lactose, maltose, manni-
tol, raffinose, rhamnose, salicin, sorbitol, sucrose, trehalose, or xylose.
Andrade’s Indicator
Composition per 100.0mL:
NaOH (1N solution).................................................................16.0mL
Acid Fuchsin.................................................................................0.1g
Preparation of Andrade’s Indicator: Add Acid Fuchsin to
NaOH solution and bring volume to 100.0mL with distilled/deionized
water.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, dulcitol, fructose, galactose, glucose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute in 10.0mL volumes
into test tubes containing inverted Durham tubes. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Add 0.5mL of sterile carbohydrate
solution to each tube.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms, particularly members of the Enterobacteriaceae. A Dur-
ham tube is used to collect gas produced during the fermentation reaction.
Acid production is indicated by a pink color.