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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Andrade Peptone Water with Meat Extract
Composition per liter:
Peptic digest of animal tissue ....................................10.0g
NaCl................................................................5.0g
Meat extract.........................................................3.0g
Andrade indicator....................................................0.1g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Caution: Acid Fuchsin in Andrade indicator is a potential carcinogen
and care must be taken to avoid inhalation of the powdered dye and
contact with the skin.
Use: With added carbohydrates, for the determination of carbohydrate
fermentation reactions of microorganisms, particularly members of the
Enterobacteriaceae. A specific carbohydrate is added to the medium to
test the fermentation of that carbohydrate. A Durham tube is used to col-
lect gas produced during the fermentation reaction. Acid production is
indicated by a pink color.