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Bismuth Sulfite Broth (m-Bismuth Sulfite Broth)

[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]

Bismuth Sulfite Broth

(m-Bismuth Sulfite Broth)

山東拓普(pu)生物(wu)工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Bi2(SO3)3...........................................................16.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

Beef extract.......................................................10.0g

Glucose .............................................................10.0g

Na2HPO4 .............................................................8.0g

FeSO4·7H2O........................................................0.6g

pH 7.7 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

boiling. Boil for 1 min. Do not autoclave. Cool to

45°–50°C. Mix to disperse the precipitate and asepti

cally distribute into sterile tubes or flasks. Use 2.0–

2.2mL of medium for each membrane filter.

Use: For the selective isolation of Salmonella typhi

and other enteric bacilli and for the detection of Sal

monella by the membrane filter method.

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