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培養基配方
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BG Sulfa Agar (Brilliant Green Sulfapyridine Agar)
[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]
BG Sulfa Agar
(Brilliant Green Sulfapyridine Agar)
山東拓普生物工程(cheng)有限公(gong)司(si) 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................20.0g
Proteose peptone No. 3.....................................10.0g
Lactose..............................................................10.0g
Sucrose..............................................................10.0g
NaCl....................................................................5.0g
Yeast extract........................................................3.0g
Sodium sulfapyridine..........................................1.0g
Brilliant Green ................................................0.125g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Heat gently to boiling. Distribute
into tubes or flasks. Autoclave for no longer than 15
min at 15 psi pressure–121°C. Pour into sterile Petri
dishes if desired.
Use: For the selective isolation of Salmonella spe
cies other than Salmonella typhi from food, dairy
products, eggs and egg products, and feed. Salmonel
la appear as red, pink, or white colonies surrounded
by zones of bright red.



