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培養基配方

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Beef Infusion Agar

[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]

Beef Infusion Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Ground defatted beef ......................................453.6g

Agar ..................................................................20.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add ground beef to 1.0L

of distilled/deionized water. Let stand overnight at 4°C.

Gently heat and bring to 80°–90°C for 60 min. Let stand

for 2 hr. Filter through muslin. To filtrate, add peptone

and salt. Mix thoroughly. Adjust pH to 7.6 with 4%

NaOH. Filter through Whatman #1 filter paper. Bring

volume of filtrate to 1.0L. Add agar. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation of a variety of microorgan

isms.

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