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Bacillus coagulans Medium

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]
Bacillus coagulans Medium

山東拓普生(sheng)物工程有限(xian)公司(si) 培(pei)養基配方(fang) //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
Glucose ...............................................................5.0g
Proteose peptone.................................................5.0g
Yeast extract........................................................5.0g
K2HPO4...............................................................4.0g
MnSO4·4H2O solution .................................. 10.0mL
CaCl2 solution............................................... 10.0mL
pH 5.0 ± 0.2 at 25°C
MnSO4·4H2O Solution:
Composition per 10.0mL:
MnSO4·4H2O .................................................0.05mg
Preparation of MnSO4·4H2O Solution: Add
MnSO4·4H2O to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
CaCl2 Solution:
Composition per 10.0mL:
CaCl2............................................................0.045mg
Preparation of CaCl2 Solution: Add CaCl2 to
distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept MnSO4·4H2O solution and CaCl2 solution, to
distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Avoid overheating. Cool to 45°–50°C. Asep
tically add sterile MnSO4·4H2O solution and CaCl2
solution. Mix thoroughly. Pour into sterile Petri dish
es or distribute into sterile tubes.
Use: For the cultivation of Bacillus coagulans.
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