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培養基配方

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Bacillus cereus Selective Agar Base

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Sodium pyruvate...............................................10.0g

Mannitol............................................................10.0g

Na2HPO4.............................................................2.5g

NaCl....................................................................2.0g

Peptone ...............................................................1.0g

KH2PO4.............................................................0.25g

Bromthymol Blue .............................................0.12g

MgSO4·7H2O......................................................0.1g

Egg yolk emulsion ........................................ 25.0mL

Polymyxin B solution ................................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Polymyxin B Solution:

Composition per 10.0mL:

Polymyxin B ..............................................100,000U

Preparation of Polymyxin B Solution: Add

Polymyxin B to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept egg yolk emulsion and plymyxin B solution, to

distilled/deionized water and bring volume to

965.0mL. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add ster

ile polymyxin B and 25.0mL of sterile egg yolk

emulsion. Mix thoroughly. Pour into sterile Petri

dishes or leave in tubes.

Use: For the selection and presumptive identifica

tion of Bacillus cereus. Also for the isolation and

enumeration of these bacteria. Bacillus cereus grows

as moderate-sized (5mm) crenated colonies, which

are turquoise, surrounded by a precipitate of egg

yolk, which is also turquoise.

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Bacillus cereus Selective Agar Base