培養基配方

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Bacillus Agar, Modified

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普(pu)生物工程有限公(gong)司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g

(NH4)2SO4 ..........................................................0.2g

Glucose ...............................................................1.0g

Yeast extract........................................................1.0g

KH2PO4...............................................................0.6g

MgSO4·7H2O......................................................0.5g

CaCl2·2H2O ......................................................0.25g

pH 3.0–4.0 at 25°C

Preparation of Medium: Add components, ex

cept agar and glucose, to distilled/deionized water

and bring volume to 500.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Adjust pH to 3.5. Pre

pare a separate agar and glucose solution by adding

20.0g of agar and 1.0g of glucose to 500.0mL of dis

tilled/deionized water. Autoclave solutions separate

ly for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C. Aseptically combine both solutions. This pro

cedure avoids acid hydrolysis of the agar. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation of acidophilic Bacillus spe

cies such as Bacillus acidocaldarius.