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Bacillus Agar

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]
Bacillus Agar

山東拓(tuo)普生物工程有限公司 培養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
(NH4)2SO4 ..........................................................1.3g
Glucose ...............................................................1.0g
Yeast extract........................................................1.0g
KH2PO4.............................................................0.37g
MgSO4·7H2O....................................................0.25g
CaCl2·2H2O ......................................................0.07g
FeCl3 .................................................................0.02g
pH 4.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to
500.0mL. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 3.5. Prepare a separate agar so
lution by adding 20.0g/500.0mL of distilled/deion
ized water. Autoclave solutions separately for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti
cally combine both solutions. This procedure avoids
acid hydrolysis of the agar. Pour into sterile Petri
dishes or leave in tubes.
Use: For the cultivation of acidophilic Bacillus spe
cies such as Bacillus acidocaldarius.
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