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培養基配方

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ALOA Medium (Agar Listeria Ottavani & Agosti) (BAM M10a)

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]

ALOA Medium

(Agar Listeria Ottavani & Agosti)

(BAM M10a)

山(shan)東拓普生物工程有限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................18.0g

Peptone .............................................................18.0g

LiCl...................................................................10.0g

Yeast extract......................................................10.0g

Tryptone..............................................................6.0g

NaCl....................................................................5.0g

Na2HPO4.............................................................2.5g

Na-pyruvate ........................................................2.0g

Glucose ...............................................................2.0g

Mg-glycerophosphate .........................................1.0g

MgSO4 ................................................................0.5g

5-Bromo4-chloro-indolyl-

β-D-glucopyranoside ..................................0.05g

Phosphatidylinositol solution........................ 50.0mL

Nalidixic acid solution.................................... 5.0mL

Ceftazidime solution....................................... 5.0mL

Cycloheximide solution.................................. 5.0mL

Polymyxin B solution ..................................... 5.0mL

pH 7.2 ± 0.2 at 25°C

Nalidixic Acid Solution:

Composition per 5.0mL:

Nalidixic acid....................................................0.02g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 5.0mL. Mix thoroughly. Filter sterilize.

Ceftazidime Solution:

Composition per 5.0mL:

Ceftazidime.......................................................0.02g

Preparation of Ceftazidime Solution: Add

ceftazidime to distilled/deionized water and bring

volume to 5.0mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 5.0mL:

Cycloheximide..................................................0.05g

Ethanol............................................................ 2.5mL

Preparation of Cycloheximide Solution: Add

cycloheximide to 2.5mL of ethanol. Mix thoroughly.

Bring volume to 5.0mL with distilled/deionized wa

ter. Filter sterilize.

Polymyxin B Solution:

Composition per 5.0mL:

Polymyxin B .................................................76700U

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 5.0mL. Mix thoroughly. Filter sterilize.

Phosphatidylinositol Solution:

Composition per 50.0mL:

L-α-phosphatidylinositol.....................................2.0g

Preparation of Phosphatidylinositol Solu

tion: Add L-α-phosphotidylinositol to cold distilled/

deionized water and bring volume to 50.0mL. Stir for

30 min so a homogeneous suspension is obtained.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 48–50°C.

Preparation of Medium: Add components, ex

cept phosphatidylinositol solution, nalidixic acid so

lution, cetazidime solution, cycloheximide solution,

and polymyxin B solution, to distilled/deionized wa

ter and bring volume to 930.0mL. Mix thoroughly.

Adjust the pH to 7.2. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 50.0mL sterile

phosphatidylinositol solution, 5.0mL sterile nalidixic

acid solution, 5.0mL sterile cetazidime solution,

5.0mL sterile cycloheximide solution, and 5.0mL

sterile polymyxin B solution. Mix thoroughly. Pour

into the Petri dishes or distribute into sterile tubes.

Use: For the isolaltion and cultivation of Listeria

spp. according to ISO standard 11290.

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ALOA Medium (Agar Listeria Ottavani & Agosti) (BAM M10a)