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培養基配方

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Allantoin Agar

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]

Allantoin Agar

山東拓普生物(wu)工程(cheng)有(you)限公司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4·12H2O ................................................9.0g

NaCl....................................................................5.0g

KH2PO4 ..............................................................1.5g

Meat extract ........................................................1.0g

Yeast extract........................................................1.0g

MgSO4·7H2O......................................................0.2g

MnCl2·4H2O ..................................................20.0mg

CaCl2................................................................1.2mg

Glucose-allantoin solution .......................... 100.0mL

Glucose-Allantoin Solution:

Composition per 100.0mL:

Glucose ...............................................................5.0g

Allantoin .............................................................1.0g

Preparation of Glucose-Allantoin Solution:

Add components to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize. Warm to 50°C.

Preparation of Medium: Add components, ex

cept glucose-allantoin solution, to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Aseptically add 100.0mL of sterile glucose-allantoin

solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the cultivation and maintenance of Bacillus

species.

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