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Alkaline Bacillus Medium

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]
Alkaline Bacillus Medium

山(shan)東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Peptone..............................................................10.0g
Glucose .............................................................10.0g
Yeast extract........................................................5.0g
K2HPO4...............................................................1.0g
Na2CO3 solution ......................................... 100.0mL
pH 8.5–11.0 at 25°C
Na2CO3 Solution:
Composition per 100.0mL:
Na2CO3 .............................................................10.0g
Preparation of Na2CO3 Solution: Add Na2CO3
to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept Na2CO3 solution, to distilled/deionized water
and bring volume to 900.0mL. Gently heat while stir
ring and bring to boiling. Autoclave for 15 min at 10
psi pressure–115°C. Cool to 45°–50°C. Aseptically
add sterile Na2CO3 solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of alkalo
philic microorganisms such as Bacillus alcalophilus,
Bacillus circulans, and other Bacillus species.
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