培養基配方

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Alginate Utilization Medium

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公(gong)司(si) 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Solution B ................................................... 500.0mL

Solution A................................................... 400.0mL

Solution C ................................................... 100.0mL

Solution A:

Composition per 400.0mL:

Marine salts.......................................................38.0g

Preparation of Solution A: Add marine salts to

distilled/deionized water and bring volume to

400.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Solution B:

Composition per 500.0mL:

Agar ..................................................................20.0g

Sodium alginate ................................................10.0g

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

500.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Solution C:

Composition per 100.0mL:

Tris·HCl buffer................................................0.067g

NaNO3.............................................................0.047g

Ferric EDTA...................................................66.5mg

Sodium glycerophosphate..............................6.67mg

Thiamine·HCl ................................................ 67.0μg

Vitamin B12 ...................................................... 1.3μg

Biotin ............................................................. 0.67μg

Preparation of Solution C: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine

solutions A, B, and C. For liquid medium, omit agar

from solution B.

Use: For the cultivation of microorganisms that can

utilize alginate as a carbon source. Growth on algi

nate (production of alginase) is a diagnostic test used

in the differentiation of Vibrio species.