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培養基配方

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Alcaligenes Medium

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]

Alcaligenes Medium

山東拓普生物工(gong)程有限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Tris....................................................................6.06g

NaCl..................................................................4.68g

KCl....................................................................1.49g

NH4Cl ...............................................................1.07g

Na2SO4..............................................................0.43g

Na2HPO4·12H2O...............................................0.23g

MgCl2·6H2O .......................................................0.2g

CaCl2·2H2O ......................................................0.03g

Ferric ammonium citrate.................................0.005g

Trace elements solution SL-7 ......................... 1.0mL

Sodium succinate solution ............................ 10.0mL

CuSO4 solution ............................................... 2.5mL

Sodium Succinate Solution:

Composition per 100.0mL:

Sodium succinate..............................................40.0g

Preparation of Sodium Succinate Solution:

Add sodium succinate to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

CuSO4 Solution:

Composition per 100.0mL:

CuSO4 ...............................................................16.0g

Preparation of CuSO4 Solution: Add CuSO4 to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Trace Elements Solution SL-7:

Composition per 1001.0mL:

CoCl2·6H2O .................................................200.0mg

MnCl2·4H2O ................................................100.0mg

ZnCl2..............................................................70.0mg

H3BO3 ............................................................60.0mg

Na2MoO4·2H2O .............................................40.0mg

CuCl2·2H2O ...................................................20.0mg

NiCl2·6H2O....................................................20.0mg

HCl (25%)....................................................... 1.0mL

Preparation of Trace Elements Solution SL-7:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, ex

cept CuSO4 solution and sodium succinate solution,

to distilled/deionized water and bring volume to

987.5mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Aseptically add 10.0mL of

sterile CuSO4 solution and 2.5mL of sterile sodium

succinate solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation of Alcaligenes species.

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Alcaligenes Medium