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培養基配方
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Alcaligenes Medium
[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]
Alcaligenes Medium
山(shan)東拓普(pu)生(sheng)物(wu)工程(cheng)有(you)限公(gong)司(si) 培(pei)養(yang)基(ji)配方 //ljxinglong.com
Composition per liter:
Peptone................................................................5.0g
Beef extract.........................................................3.0g
Ferric citrate........................................................0.2g
Ammonium lactate solution............................ 3.0mL
pH 7.0 ± 0.2 at 25°C
Ammonium Lactate Solution:
Composition per 100.0mL:
Lactic acid.........................................................60.0g
Preparation of Ammonium Lactate Solu
tion: Dissolve lactic acid in 100.0mL of distilled/
deionized water. Neutralize with NH4OH to pH 7.0.
Preparation of Medium: Add peptone, beef ex
tract, and ammonium lactate to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Add ferric citrate
aseptically. Mix thoroughly. Aseptically distribute
into tubes or flasks.
Use: For the cultivation of Alcaligenes tolerans.



