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Aeromonas Differential Agar (Dextrin Fuchsin Sulfite Agar)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]

Aeromonas Differential Agar
(Dextrin Fuchsin Sulfite Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Dextrin ..............................................................15.0g
Agar ..................................................................13.0g
Pancreatic digest of casein................................10.0g
Na2HPO4...........................................................7.75g
NaCl....................................................................5.0g
Beef extract.........................................................3.0g
Na2SO3................................................................1.6g
Acid Fuchsin solution................................... 50.0mL
pH 7.5 ± 0.2 at 25°C
Acid Fuchsin Solution:
Composition per 50.0mL:
Acid Fuchsin.....................................................0.25g
Preparation of Acid Fuchsin Solution: Add
Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix
well to dissolve.
Caution: Acid Fuchsin is a potential carcinogen
and care must be taken to avoid inhalation of the
powdered dye and contamination of the skin.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes.
Use: For the isolation and differentiation of Aeromo
nas species from other Gram-negative rods such as
Pseudomonas and Enterobacteriaceae. Specimens
with low numbers of Aeromonas may first be en
riched by growth in starch broth for 4–9 days. After
24 hrs of growth on this agar, colonies are sprayed
with Nadi reagent (1% solution of N,N,N′,N′-tetram
ethyl-p-phenylene-diammonium dichloride). A posi
tive Nadi reaction (dextrin degradation) is indicated
by a purple color at the periphery of the colony. Dex
trin fermentation is also indicated by red colonies.
Aeromonas species appear as large, convex, dark red
colonies with a purple periphery.

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