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培養基配方

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AE Sporulation Medium, Modified (BAM M5)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]

AE Sporulation Medium, Modified

(BAM M5)

山(shan)東拓(tuo)普生物工程有限公司(si) 培養基配方 //ljxinglong.com

Composition per 1079.2mL:

Polypeptone™ ..................................................10.0g

Yeast extract......................................................10.0g

Na2HPO4 ..........................................................4.36g

Ammonium acetate.............................................1.5g

KH2PO4 ............................................................0.25g

MgSO4·7H2O ......................................................0.2g

Raffinose solution ......................................... 39.6mL

Na2CO3 solution ........................................... 13.2mL

CoCl2·6H2O solution .................................... 13.2mL

Sodium ascorbate solution ............................ 13.2mL

pH 7.8 ± 0.1 at 25°C

Raffinose Solution:

Composition per 100.0mL:

Raffinose...........................................................10.0g

Preparation of Raffinose Solution: Add raffi

nose to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Na2CO3 Solution:

Composition per 100.0mL:

Na2CO3 ...............................................................7.0g

Preparation of Na2CO3 Solution: Add Na2CO3

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

CoCl2 Solution:

Composition per 100.0mL:

CoCl2·6H2O......................................................0.32g

Preparation of CoCl2 Solution: Add

CoCl2·6H2O to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Sodium Ascorbate Solution:

Composition per 100.0mL:

Sodium ascorbate................................................1.5g

Preparation of Sodium Ascorbate Solution:

Add sodium ascorbate to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize. Use freshly prepared solution.

Preparation of Medium: Add components—ex

cept raffinose solution, Na2CO3 solution, CoCl2 so

lution, and sodium ascorbate solution—to distilled/

deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 7.5 using 2m sodium carbonate

solution. Distribute into tubes in 15.0mL volumes.

Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically add 0.6mL of sterile raffinose solution,

0.2mL of sterile Na2CO3 solution, and 0.2mL of ster

ile CoCl2 solution to each tube. Mix thoroughly. Pri

or to inoculation, steam medium for 10 min. Cool to

25°C. Aseptically add 0.2mL of sterile sodium ascor

bate solution to each tube.

Use: For the cultivation and sporulation of Clostrid

ium perfringens.

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AE Sporulation Medium, Modified (BAM M5)