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ABY Agar (Acid Bismuth Yeast Agar)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
ABY Agar
(Acid Bismuth Yeast Agar)

山東拓普(pu)生物(wu)工(gong)程有限公(gong)司 培養基配方(fang) //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
Glucose .............................................................20.0g
Bi2(SO3)2.............................................................8.0g
(NH4)2SO4...........................................................3.0g
KH2PO4...............................................................3.0g
MgSO4·7H2O ....................................................0.25g
CaCl2·2H2O.......................................................0.25g
Biotin ............................................................. 10.0μg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool tubes in a slanted po
sition.
Use: For the selective isolation and differentiation of
Candida albicans, which can cause food allergies,
from other Candida species. Candida albicans and
Candida tropicalis colonies appear as smooth,
brownish-black round colonies. Other Candida spe
cies are differentially pigmented or produce diffus
ible pigments. Usually used in conjunction with BiG
GY agar to differentiate further Candida; on BiGGY
agar, Candida albicans appears as brown to black
colonies with no pigment diffusion and no sheen,
whereas Candida tropicalis appears as dark brown
colonies with black centers, black pigment diffusion,
and a sheen.
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