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培養基配方

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Abeyta-Hunt Bark Agar (BAM M29a)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]

Abeyta-Hunt Bark Agar

(BAM M29a)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1016mL:

Beef heart, infusion from................................500.0g

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Horse blood, lysed ........................................ 50.0mL

Cefoperazone solution .................................... 4.0mL

Rifampicin solution ........................................ 4.0mL

Amphotericin B solution................................. 4.0mL

Ferrous sulfate pyruvate

metabisulfite solution ............................... 4.0mL

pH 7.4 ± 0.2 at 25°C

Amphotericin B Solution:

Composition per 100mL:

Amphotericin B ................................................0.05g

Preparation of Amphotericin B Solution:

Add Amphotericin B to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Cefoperazone Solution:

Composition per 100mL:

Sodium cefoperazone........................................0.08g

Preparation of Cefoperazone Solution: Add

sodium cefoperazone to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Rifampicin Solution:

Composition per 100mL:

Rifampicin ........................................................0.25g

Preparation of Rifampicin Solution: Add

rifampicin to 70.0mL ethanol. Mix thoroughly. Add

distilled/deionized water to bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Ferrous Sulfate, Pyruvate, Metabisulfite So

lution:

Composition per 100mL:

FeSO4................................................................6.25g

Na-pyruvate ......................................................6.25g

Na-metabisulfite ...............................................6.25g

Preparation of Ferrous Sulfate, Pyruvate,

Metabisulfite Solution: Add Na-pyruvate to

20mL distilled/deionized water. Mix thorougly. Add

Na-metabisulfite and FeSO4. Bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept cefoperazone solution, amphotericin B solution,

rifampicin solution, ferrous sulfate pyruvate met

abisulfide solution, and horse blood, to distilled/

deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°C. Aseptically add 4.0mL cefoperazone solution,

4.0mL amphotericin B solution, 4.0mL rifampicin

solution, 4.0mL ferrous sulfate pyruvate metabisul-

fide solution, and 50.0mL lysed horse blood. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation and cultivation of Campylo

bacter spp.

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Abeyta-Hunt Bark Agar (BAM M29a)