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Zymobacterium Broth

[所屬分類:培養基配方] [發布時間:2021-10-25] [發布人:網站管理員2] [閱讀次數:] [返回]

Zymobacterium Broth

山東拓普(pu)生(sheng)物工程(cheng)有限公司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Orotic acid..................................................................................... 2.0g

Na3PO4·12H2O.............................................................................. 1.5g

Sodium thioglycolate .................................................................... 1.0g

NaOH ............................................................................................ 0.5g

Riboflavin ................................................................................. 0.015g

pH 7.9 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa

ter in the following order: tryptone, orotic acid, sodium hydroxide, sodium

phosphate, sodium thioglycolate, riboflavin and bring volume to 1.0L.

Mix thoroughly. Some orotic acid will remain undissolved. Adjust pH to

7.9 using NaH2PO4. Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Mix again to dissolve orotic acid.

Use: For the cultivation and maintenance of Clostridium (Zymobacte

rium) oroticum.