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Zymobacterium Agar

[所屬分類:培養基配方] [發布時間:2021-10-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Zymobacterium Agar

山東拓普生物(wu)工程有限公司 培養基(ji)配方(fang) //ljxinglong.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g
Agar ............................................................................................ 15.0g
Orotic acid..................................................................................... 2.0g
Na3PO4·12H2O.............................................................................. 1.5g
Sodium thioglycolate .................................................................... 1.0g
NaOH ............................................................................................ 0.5g
Riboflavin ................................................................................. 0.015g
pH 7.9 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa
ter in the following order: tryptone, orotic acid, sodium hydroxide, sodium
phosphate, sodium thioglycolate, riboflavin, agar and bring volume to
1.0L . Mix thoroughly. Some orotic acid will remain undissolved. Adjust
pH to 7.9 using NaH2PO4.Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Mix thoroughly to dissolve orotic acid. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Clostridium (Zymobacte
rium) oroticum.
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