培養基配方

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YPSC Agar, Cation-Supplemented

[所屬分類:培養基配方] [發布時間:2021-10-25] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Sodium acetate·3H2O.................................................................. 50.0g

Agar ............................................................................................ 15.0g

Peptone ....................................................................................... 10.0g

Yeast extract................................................................................ 10.0g

MgSO4·7H2O.............................................................................. 0.74g

CaCl2·2H2O ................................................................................ 0.29g

L-Cysteine·HCl·H2O ................................................................... 0.05g

Bacitracin solution...................................................................10.0mL

Bacitracin Solution:

Composition per 10.0mL:

Bacitracin................................................................................. 6,000U

Preparation of Bacitracin Solution: Add bacitracin to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except bacitracin solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bac

itracin solution. Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation and enumeration of Bdellovibrio species.