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培養基配方

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YP87 Medium

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]

YP87 Medium

山(shan)東拓普生物工程有限公司(si) 培養基配方(fang) //ljxinglong.com

Composition per liter:

Na2SO4.......................................................................................... 4.0g

NaHCO3........................................................................................ 1.3g

KCl................................................................................................ 0.5g

Yeast extract.................................................................................. 0.5g

MgCl2·6H2O ................................................................................. 0.4g

NH4Cl ......................................................................................... 0.25g

L-Ascorbic acid............................................................................. 0.2g

Na2HPO4 ...................................................................................... 0.2g

Sodium thioglycolate.................................................................... 0.2g

CaCl2·2H2O ................................................................................ 0.15g

Resazurin ................................................................................... 1.0mg

Modified Wolfe’s mineral solution..........................................10.0mL

Wolfe’s vitamin solution..........................................................10.0mL

Sodium lactate, 60% syrup ........................................................3.0mL

pH 7.5 ± 0.2 at 25°C

Modified Wolfe’s Mineral Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·H2O.................................................................................. 0.5g

CaCl2............................................................................................. 0.1g

CoCl2·6H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

AlK(SO4)2·12H2O....................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O .......................................................................... 0.01g

Na2SeO3 ...................................................................................... 0.01g

NaWO4·2H2O.............................................................................. 0.01g

NiC12·6H2O ................................................................................ 0.01g

Preparation of Modified Wolfe’s Mineral Solution: Add

nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH

to 6.5 with KOH. Add remaining components one at a time. Add dis

tilled/deionized water to 1.0L. Adjust pH to 6.8.

Wolfe’s Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................10.0mg

p-Aminobenzoic acid.................................................................5.0mg

Lipoic acid .................................................................................5.0mg

Nicotinic acid.............................................................................5.0mg

Riboflavin ..................................................................................5.0mg

Thiamine·HCl ............................................................................5.0mg

Calcium DL-pantothenate...........................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid....................................................................................2.0mg

Vitamin B12 ................................................................................0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 100%

N2. Add components, except L-ascorbic acid, NaHCO3, and sodium thio

glycolate, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH to 7.5. Gently heat and bring to boiling. Cool

while sparging with 100% N2. Add L-ascorbic acid, NaHCO3, and sodi

um thioglycolate. Mix thoroughly. Sparge with 100% N2. Distribute into

tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thermodesulfovibrio yellowstonii.

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YP87 Medium