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培養基配方
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Yopp’s Medium

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]
Yopp’s Medium

山東拓(tuo)普生(sheng)物工(gong)程有限公(gong)司 培養(yang)基(ji)配方 //ljxinglong.com

Composition per liter:

NaCl........................................................................................ 116.88g
MgCl2·6H2O ............................................................................. 10.68g
MgSO4·7H2O.............................................................................. 10.0g
KCl................................................................................................ 2.0g
CaNO3·4H2O ................................................................................ 1.0g
Glycyl-glycine buffer.................................................................... 0.5g
K2HPO4·3H2O .......................................................................... 0.065g
Ferric EDTA .............................................................................. 5.0mg
Trace metals solution .................................................................1.0mL
pH 7.8 ± 0.2 at 25°C
Trace Metals Solution:
Composition per liter:
MnCl2·4H2O ................................................................................. 2.0g
H3BO3 ........................................................................................... 0.5g
ZnNO3·6H2O ................................................................................ 0.5g
Co(NO3)2·6H2O ........................................................................ 0.025g
CuCl2·2H2O .............................................................................. 0.025g
Na2MoO4·2H2O ........................................................................ 0.025g
VOSO4·6H2O............................................................................ 0.025g
HCl.............................................................................................3.0mL
Preparation of Trace Metals Solution: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the isolation and cultivation of halophilic cyanobacteria.
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