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培養基配方

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Yeast Nitrogen Base

[所屬分類:培養基配方] [發布時間:2021-10-20] [發布人:網站管理員2] [閱讀次數:] [返回]

Yeast Nitrogen Base

山東(dong)拓普生物工程(cheng)有限公司培養基配方(fang) //ljxinglong.com

Composition per liter:

(NH4)2SO4..................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

NaCl .............................................................................................. 0.1g

CaCl2·2H2O................................................................................... 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol .......................................................................................2.0mg

KI ...............................................................................................1.0mg

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

MnSO4·4H2O .............................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

Pyroxidine·HCl ..........................................................................0.4mg

Niacin.........................................................................................0.4mg

Calcium pantothenate ................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

Riboflavin ..................................................................................0.2mg

FeCl3 ..........................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Alternately

for carbon assimilation tests, prepare a 10X concentrated solution by

adding components to distilled/deionized water and bringing volume

to 100.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Prepare a carbohydrate solution

by adding 0.5g of carbohydrate to 90.0mL of distilled/deionized water.

Mix thoroughly. Filter sterilize. Aseptically add 0.5mL of the 10X con

centrated solution to 4.5mL of the filter-sterilized carbohydrate solu

tion. Mix thoroughly.

Use: For carbohydrate assimilation tests in the characterization and

identification of yeasts.

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Yeast Nitrogen Base