培養基配方

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Yeast Morphology Agar

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山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

(NH4)2SO4 .................................................................................. 32.5g

Agar ............................................................................................ 18.0g

Glucose ....................................................................................... 10.0g

Asparagine .................................................................................... 1.5g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.5g

CaCl2·2H2O .................................................................................. 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol .......................................................................................2.0mg

H3BO3 ........................................................................................0.5mg

Calcium pantothenate ................................................................0.4mg

MgSO4·7H2O .............................................................................0.4mg

Niacin.........................................................................................0.4mg

Pyridoxine·HCl ..........................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

ZnSO4·7H2O ..............................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

FeCl3 ..........................................................................................0.2mg

Riboflavin ..................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

KI ...............................................................................................0.1mg

CuSO4·5H2O ............................................................................0.04mg

Biotin ..........................................................................................2.0μg

Folic acid.....................................................................................2.0μg

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For agar dilution and diffusion disk testing with 5-flucytosine.

For the microbiological assay of flucytosine using Candida kefyr

ATCC 28838 or Saccharomyces cerevisiae ATCC 36375 as the indi

cator microorganism.

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