培養基配方

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Yeast Fermentation Medium

[所屬分類:培養基配方] [發布時間:2021-10-20] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公(gong)司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Peptone.......................................................................................... 7.5g

Yeast extract.................................................................................. 4.5g

Bromthymol Blue (1.6% solution).............................................1.0mL

Carbohydrate solution................................................................1.0mL

Carbohydrate Solution:

Composition per 10.0mL:

Carbohydrate................................................................................. 0.6g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 10.0mL. Glucose, maltose,

lactose, galactose, or trehalose may be used. If raffinose is used, prepare

a 12% solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except carbohydrate

solution, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distribute in 2.0mL vol

umes into test tubes that contain an inverted Durham tube. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 1.0mL of sterile carbohydrate solution. Mix thoroughly.

Use: For the cultivation and differentiation of yeast based on carbohy

drate fermentation patterns. Yeasts that can ferment a specific carbo

hydrate turn the medium yellow.

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