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Yeast Fermentation HiVeg Broth Base with Carbohydrate (Bromcresol Purple HiVeg Broth Base)

[所屬分類:培養基配方] [發布時間:2021-10-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Fermentation HiVeg Broth Base
with Carbohydrate
(Bromcresol Purple HiVeg Broth Base)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Plant peptone............................................................................... 10.0g
NaCl .............................................................................................. 5.0g
Plant extract .................................................................................. 3.0g
Bromcresol Purple ...................................................................... 0.04g
Carbohydrate solution..............................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without carbohydrate solution, is available as
a premixed powder from HiMedia.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate............................................................................... 10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute in 10.0mL vol
umes into test tubes containing inverted Durham tubes. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Add 0.5mL of sterile
carbohydrate solution to each tube.
Use: For the determination of carbohydrate fermentation reactions of
microorganisms.
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