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YC Medium without Tryptophan

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
YC Medium without Tryptophan

山東(dong)拓普生物工程有限公司(si) 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Solution A..............................................................................500.0mL
Solution B ..............................................................................500.0mL
Solution A:
Composition per 500.0mL:
Glucose ....................................................................................... 20.0g
Succinic acid............................................................................... 10.0g
NaOH............................................................................................ 6.0g
(NH4)2SO4 .................................................................................... 5.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O................................................................................ 0.5g
NaCl.............................................................................................. 0.1g
CaCl2·2H2O .................................................................................. 0.1g
Adenine......................................................................................... 0.1g
L-Arginine..................................................................................... 0.1g
L-Cysteine ..................................................................................... 0.1g
L-Leucine ...................................................................................... 0.1g
L-Lysine ........................................................................................ 0.1g
L-Threonine................................................................................... 0.1g
Uracil ............................................................................................ 0.1g
L-Aspartic acid............................................................................ 0.05g
L-Histidine .................................................................................. 0.05g
L-Isoleucine................................................................................. 0.05g
L-Methionine............................................................................... 0.05g
L-Phenylalanine .......................................................................... 0.05g
L-Proline ..................................................................................... 0.05g
L-Serine....................................................................................... 0.05g
L-Tyrosine ................................................................................... 0.05g
L-Valine....................................................................................... 0.05g
Inositol ....................................................................................... 2.0mg
KI ............................................................................................... 1.0mg
H3BO3 ........................................................................................ 0.5mg
ZnSO4·7H2O .............................................................................. 0.4mg
MnSO4·4H2O............................................................................. 0.4mg
Thiamine·HCl ............................................................................ 0.4mg
Pyroxidine·HCl .......................................................................... 0.4mg
Niacin......................................................................................... 0.4mg
Calcium pantothenate ................................................................ 0.4mg
p-Aminobenzoic acid................................................................. 0.2mg
Riboflavin .................................................................................. 0.2mg
FeCl3 .......................................................................................... 0.2mg
Na2MoO4·4H2O ......................................................................... 0.2mg
CuSO4·5H2O ............................................................................0.04mg
Folic acid.....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Adjust pH to 5.6–5.7. Filter ster
ilize. Warm to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar, noble.................................................................................. 20.0g
Preparation of Solution B: Add agar to distilled/deionized water
and bring volume to 500.0mL. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 50–55°C.
Preparation of Medium: Aseptically combine 500.0mL of solu
tion A and 500.0mL of solution B. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the cultivation of Saccharomyces cerevisiae.
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