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YC Agar without Tryptophan

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
YC Agar without Tryptophan

山(shan)東拓(tuo)普(pu)生物工程有限公司 培養基配方(fang) //ljxinglong.com

Composition per liter:

Solution A ..............................................................................500.0mL
Solution B ..............................................................................500.0mL
Solution A:
Composition per 500.0mL:
Glucose ....................................................................................... 20.0g
Succinic acid ............................................................................... 10.0g
NaOH ............................................................................................ 6.0g
(NH4)2SO4 .................................................................................... 5.0g
KH2PO4......................................................................................... 0.6g
MgSO4·7H2O ................................................................................ 0.3g
Adenine......................................................................................... 0.1g
L-Arginine ..................................................................................... 0.1g
L-Cysteine ..................................................................................... 0.1g
L-Leucine ...................................................................................... 0.1g
L-Lysine......................................................................................... 0.1g
L-Threonine................................................................................... 0.1g
Uracil ............................................................................................ 0.1g
L-Aspartic acid............................................................................ 0.05g
L-Histidine................................................................................... 0.05g
L-Isoleucine................................................................................. 0.05g
L-Methionine............................................................................... 0.05g
L-Phenylalanine........................................................................... 0.05g
L-Proline...................................................................................... 0.05g
L-Serine....................................................................................... 0.05g
L-Tyrosine ................................................................................... 0.05g
L-Valine....................................................................................... 0.05g
NaCl ............................................................................................ 0.05g
CaCl2·2H2O................................................................................. 0.05g
DL-Methionine............................................................................. 0.01g
L-Histidine·HCl ......................................................................... 0.005g
Inositol .......................................................................................1.0mg
KI ...............................................................................................0.5mg
H3BO3 ........................................................................................0.3mg
ZnSO4·7H2O ..............................................................................0.2mg
MnSO4·4H2O .............................................................................0.2mg
Thiamine·HCl ............................................................................0.2mg
Pyroxidine·HCl ..........................................................................0.2mg
Niacin.........................................................................................0.2mg
Calcium pantothenate ................................................................0.2mg
p-Aminobenzoic acid.................................................................0.1mg
Riboflavin ..................................................................................0.1mg
FeCl3 ..........................................................................................0.1mg
Na2MoO4·4H2O .........................................................................0.1mg
CuSO4·5H2O ............................................................................0.02mg
Folic acid.....................................................................................1.0μg
Biotin ..........................................................................................1.0μg
pH 5.7 ± 0.2 at 25°C
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to
5.6–5.7. Filter sterilize. Warm to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar, noble.................................................................................. 20.0g
Preparation of Solution B: Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically mix 500.0mL of solution A
and 500.0mL of solution B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Saccharomyces cerevi
siae.
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