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Y 1 Adrenal Cell Growth Medium
[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Y 1 Adrenal Cell Growth Medium
山東拓(tuo)普(pu)生物工程有(you)限公司 培(pei)養基配方 //ljxinglong.com
Composition per 580.0mL:
Ham’s F-10 medium ..............................................................500.0mL
Fetal bovine serum...................................................................75.0mL
Penicillin-streptomycin solution ................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Ham’s F-10 Medium:
Composition per liter:
NaCl .............................................................................................. 7.4g
NaHCO3 ........................................................................................ 1.2g
Glucose ......................................................................................... 1.1g
NaH2PO4·H2O............................................................................. 0.29g
KCl.............................................................................................. 0.28g
L-Arginine·HCl............................................................................ 0.21g
L-Glutamine................................................................................. 0.15g
MgSO4·7H2O .............................................................................. 0.15g
Sodium pyruvate ......................................................................... 0.11g
KH2PO4....................................................................................... 0.08g
CaCl2·2H2O................................................................................. 0.04g
L-Cystine·2HCl............................................................................ 0.04g
L-Histidine·HCl·H2O................................................................... 0.02g
L-Lysine·HCl ............................................................................... 0.02g
L-Asparagine-H2O....................................................................... 0.01g
L-Aspartic Acid ........................................................................... 0.01g
L-Glutamic acid ........................................................................... 0.01g
L-Leucine..................................................................................... 0.01g
L-Proline...................................................................................... 0.01g
L-Serine ....................................................................................... 0.01g
L-Alanine....................................................................................8.9mg
Glycine.......................................................................................7.5mg
D-Phenylalanine..........................................................................5.0mg
L-Methionine ..............................................................................4.5mg
Hypoxanthine.............................................................................4.1mg
L-Threonine ................................................................................3.6mg
L-Valine ..................................................................................... 3.5mg
L-Isoleucine ................................................................................2.6mg
L-Tyrosine...................................................................................1.8mg
Vitamin B12 ................................................................................1.4mg
Folic acid....................................................................................1.3mg
Phenol Red.................................................................................1.2mg
Thiamine·HCl ............................................................................1.0mg
FeSO4·7H2O...............................................................................0.8mg
Choline chloride.........................................................................0.7mg
D-Calcium pantothenate .............................................................0.7mg
Thymidine..................................................................................0.7mg
Niacinamide ...............................................................................0.6mg
L-Tryptophan ..............................................................................0.6mg
Isoinositol...................................................................................0.5mg
Riboflavin ..................................................................................0.4mg
Lipoic acid .................................................................................0.2mg
Pyridoxine·HCl ..........................................................................0.2mg
ZnSO4·7H2O ............................................................................0.03mg
Biotin .......................................................................................0.02mg
CuSO4·5H2O ...............................................................................3.0μg
Preparation of Ham’s F-10 Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Penicillin-Streptomycin Solution:
Composition per 100.0mL:
Streptomycin................................................................................. 0.5g
Penicillin G .......................................................................... 500,000U
Preparation of Penicillin-Streptomycin Solution: Add compo
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine components. Filter
sterilize. Store at 4°–5°C.
Use: For the cultivation of Y-1 mouse adrenal tissue culture cells used for
the detection of cholera enterotoxin (CT) produced by enterotoxigenic
strains of Vibrio cholerae or Vibrio mimicus. CT causes the conversion of
elongated fibroblast-like cells into round, refractile cells.
