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培養基配方

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Y 1 Adrenal Cell Growth Medium

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓(tuo)普(pu)生物工(gong)程有限公司培養基配方 //ljxinglong.com

Composition per 101.0mL:

Ham’s F-10 medium ................................................................90.0mL

Fetal bovine serum...................................................................10.0mL

Penicillin-streptomycin solution ................................................1.0mL

pH 7.0 ± 0.2 at 25°C

Ham’s F-10 Medium:

Composition per liter:

NaCl.............................................................................................. 7.4g

NaHCO3........................................................................................ 1.2g

Glucose ......................................................................................... 1.1g

NaH2PO4·H2O............................................................................. 0.29g

KCl.............................................................................................. 0.28g

L-Arginine·HCl............................................................................ 0.21g

L-Glutamine ................................................................................ 0.15g

MgSO4·7H2O.............................................................................. 0.15g

Sodium pyruvate......................................................................... 0.11g

KH2PO4....................................................................................... 0.08g

CaCl2·2H2O ................................................................................ 0.04g

L-Cystine·2HCl ........................................................................... 0.04g

L-Histidine·HCl·H2O................................................................... 0.02g

L-Lysine·HCl ............................................................................... 0.02g

L-Asparagine-H2O....................................................................... 0.01g

L-Aspartic Acid ........................................................................... 0.01g

L-Glutamic acid........................................................................... 0.01g

L-Leucine .................................................................................... 0.01g

L-Proline...................................................................................... 0.01g

L-Serine ....................................................................................... 0.01g

L-Alanine.................................................................................... 8.9mg

Glycine....................................................................................... 7.5mg

D-Phenylalanine ......................................................................... 5.0mg

L-Methionine.............................................................................. 4.5mg

Hypoxanthine............................................................................. 4.1mg

L-Threonine................................................................................ 3.6mg

L-Valine ..................................................................................... 3.5mg

L-Isoleucine................................................................................ 2.6mg

L-Tyrosine .................................................................................. 1.8mg

Vitamin B12 ................................................................................ 1.4mg

Folic acid ................................................................................... 1.3mg

Phenol Red................................................................................. 1.2mg

Thiamine·HCl ............................................................................ 1.0mg

FeSO4·7H2O............................................................................... 0.8mg

Choline chloride......................................................................... 0.7mg

D-Calcium pantothenate ............................................................. 0.7mg

Thymidine.................................................................................. 0.7mg

Niacinamide............................................................................... 0.6mg

L-Tryptophan.............................................................................. 0.6mg

Isoinositol .................................................................................. 0.5mg

Riboflavin .................................................................................. 0.4mg

Lipoic acid ................................................................................. 0.2mg

Pyridoxine·HCl .......................................................................... 0.2mg

ZnSO4·7H2O ............................................................................ 0.03mg

Biotin ....................................................................................... 0.02mg

CuSO4·5H2O...............................................................................3.0μg

Preparation of Ham’s F-10 Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Penicillin-Streptomycin Solution:

Composition per 100.0mL:

Streptomycin................................................................................. 0.5g

Penicillin G .......................................................................... 500,000U

Preparation of Penicillin-Streptomycin Solution: Add compo

nents to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine components. Filter

sterilize. Store at 4–5°C.

Use: For the cultivation of Y-1 mouse adrenal tissue culture cells used

for the detection of heat-labile toxin (LT) produced by enterotoxigenic

strains of Escherichia coli. LT causes the conversion of elongated

fibroblast-like cells into round, refractile cells.

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Y 1 Adrenal Cell Growth Medium