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培養基配方
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Xylella fastidiosa Medium (LMG 115)
[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Xylella fastidiosa Medium
(LMG 115)
山東拓普生物工(gong)程有限公司 培養(yang)基配(pei)方 //ljxinglong.com
Composition per liter:
Agar ............................................................................................ 17.0g
Yeast extract................................................................................ 10.0g
ACES buffer................................................................................ 10.0g
Activated charcoal ........................................................................ 2.0g
L-Cysteine-iron solution...........................................................20.0mL
pH 6.9 ± 0.2 at 25°C
L-Cysteine-Iron Solution:
Composition per 20.0mL:
L-Cysteine·HCl.............................................................................. 0.4g
Fe4(P2O7)3 ................................................................................... 0.25g
Preparation of L-Cysteine-Iron Solution: Add components to
distilled/deionized water and bring volume to 20.0mL. Mix thorough
ly. Filter sterilize.
Preparation of Medium: Add ACES to 500.0mL of distilled/de
ionized water at 50°C. Add a solution containing 40.0mL of 1N KOH
in 440.0mL of distilled water. Mix thoroughly. Add the remaining
components, except L-cysteine-iron solution. Mix thoroughly. Gently
heat and bring to boiling. Adjust pH to 6.9 with KOH. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add
20.0mL of sterile L-cysteine-iron solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Xylella fastidiosa.



