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Xylella Agar (LMG Medium 115)

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Xylella Agar
(LMG Medium 115)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 17.0g
Yeast extract................................................................................ 10.0g
ACES .......................................................................................... 10.0g
Activated charcoal ........................................................................ 2.0g
α-ketoglutarate.............................................................................. 1.0g
KOH, 1N.....................................................................................40mL
L-Cysteine-iron solution ..........................................................20.0mL
pH 6.9 ± 0.2 at 25°C
L-Cysteine-Iron Solution:
Composition per 20.0mL:
L-Cysteine·HCl.............................................................................. 0.4g
Fe4(P2O7)3 ................................................................................... 0.25g
Preparation of L-Cysteine-Iron Solution: Add components to
distilled/deionized water and bring volume to 20.0mL. Mix thorough
ly. Filter sterilize.
Preparation of Medium: Add ACES to 500.0mL of distilled water
at 50°C. Combine with a solution containing 40.0mL of 1N KOH in
440.0mL of distilled water. Add the other components except cysteine
iron solution. Mix thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 20.0mL sterile cysteine iron solution. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Xylella spp.
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