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培養基配方

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XPS Agar

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]

XPS Agar

山東拓普生(sheng)物(wu)工程有限公(gong)司培養(yang)基(ji)配方 //ljxinglong.com

Composition per liter:

Solution A ..............................................................................500.0mL

Solution B ..............................................................................500.0mL

pH 5.1 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

Potatoes, infusion from ............................................................... 40.0g

Sucrose........................................................................................ 15.0g

Peptone.......................................................................................... 5.0g

Glucose ......................................................................................... 4.0g

Casamino acids ............................................................................. 1.0g

Na2HPO4 ..................................................................................... 0.79g

Ca(NO3)2·4H2O solution..........................................................10.0mL

Potatoes, Infusion From:

Composition per 500.0mL:

Potatoes......................................................................................... 4.0g

Preparation of Potatoes, Infusion From: Peel and dice potatoes.

Add 400.0mL of distilled/deionized water. Gently heat and bring to

boiling. Continue boiling for 30 min. Filter through cheesecloth.

Ca(NO3)2·4H2O Solution:

Composition per 10.0mL:

Ca(NO3)2·4H2O............................................................................. 0.5g

Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Solution A: Add components, except Ca(NO3)2·4H2O

solution, to distilled/deionized water and bring volume to 490.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep

tically add 10.0mL of sterile Ca(NO3)2·4H2O solution. Mix thorough

ly. Cool to 50°–55°C.

Solution B:

Composition per 500.0mL:

Agar ............................................................................................ 20.0g

Preparation of Solution A: Add agar to distilled/deionized water

and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Preparation of Medium: Aseptically combine 500.0mL of solu

tion A with 500.0mL of solution B. Mix thoroughly. Aseptically pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Xanthomonas campestris.

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XPS Agar