培養基配方

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XLT4 HiVeg Agar Base

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公(gong)司培養基(ji)配方(fang) //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 18.0g

Lactose.......................................................................................... 7.5g

Saccharose .................................................................................... 7.5g

Na2S2O3 ........................................................................................ 6.8g

L-Lysine ........................................................................................ 5.0g

NaCl.............................................................................................. 5.0g

Xylose......................................................................................... 3.75g

Yeast extract.................................................................................. 3.0g

Plant peptone No. 3....................................................................... 1.6g

Ferric ammonium citrate............................................................... 0.8g

Phenol Red.................................................................................. 0.08g

Selective supplement solution ...................................................4.6mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without selective supplement solution, is avail

able as a premixed powder from HiMedia.

Selective Supplement Solution:

Composition per 100.0mL:

Tergitol™ 4........................................................................ Proprietary

Preparation of Selective Supplement Solution: Available as pre

mixed solution.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Do not overheat. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or

leave in tubes. Plates should be poured as soon as possible to avoid pre

cipitation.

Use: For the isolation and differentiation of enteric pathogens, espe

cially Shigella and Providencia species.