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Xanthobacter agilis Agar

[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]
Xanthobacter agilis Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1100.0mL:

Solution A.....................................................................................1.0L
Solution B ..............................................................................100.0mL
Solution A:
Composition per liter:
Agar ............................................................................................ 15.0g
NaH2PO4·12H2O........................................................................... 9.0g
KH2PO4......................................................................................... 1.5g
NH4·Cl .......................................................................................... 1.0g
Sodium propionate or 3-hydroxybutyrate..................................... 1.0g
MgSO4·7H2O ................................................................................ 0.2g
Trace elements solution .............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 2.0mL:
H3BO4 .....................................................................................560.0μg
ZnSO4·7H2O ...........................................................................350.0μg
NiCl2·H2O ...............................................................................160.0μg
Na2MoO4·2H2O ......................................................................100.0μg
CuSO4·5H2O .............................................................................16.0μg
MnCl2·4H2O..............................................................................16.0μg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 2.0mL. Mix thoroughly.
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Solution B:
Composition per 100.0mL:
Ferric ammonium citrate..........................................................50.0mg
CaCl2·2H2O............................................................................100.0mg
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Preparation of Medium: Aseptically combine 1.0L of sterile solu
tion A with 100.0mL of sterile solution B. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Xanthobacter agilis.
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