培養基配方

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Xanthine Agar

[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]

Xanthine Agar

山(shan)東(dong)拓普生物工程有限公(gong)司培養基配方(fang) //ljxinglong.com

Composition per liter:

Solution 1...............................................................................900.0mL

Solution 2...............................................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Solution 1:

Composition per 900.0mL:

Agar ............................................................................................ 15.0g

Pancreatic digest of gelatin........................................................... 5.0g

Beef extract................................................................................... 3.0g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring

to boiling.

Solution 2:

Composition per 100.0mL:

Xanthine........................................................................................ 4.0g

Preparation of Solution 2: Add xanthine to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Gently heat and

bring to boiling.

Preparation of Medium: Combine solutions 1 and 2. Mix thor

oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of aerobic Actinomycete species. Clearing

around a colony indicates utilization of xanthine. Streptomyces species

utilize xanthine; most Nocardia and Actinomadura species do not uti

lize xanthine.