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培養基配方
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WL Nutrient Broth (Wallerstein Laboratory Nutrient Broth)
[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]
WL Nutrient Broth
(Wallerstein Laboratory Nutrient Broth)
山東拓(tuo)普生物工程有限公司(si) 培(pei)養(yang)基配方 //ljxinglong.com
Composition per liter:
Glucose ....................................................................................... 50.0g
Pancreatic digest of casein............................................................ 5.0g
Yeast extract.................................................................................. 4.0g
KH2PO4....................................................................................... 0.55g
KCl............................................................................................ 0.425g
CaCl2......................................................................................... 0.125g
MgSO4·7H2O ............................................................................ 0.125g
Bromcresol Green ..................................................................... 0.022g
FeCl3 ..........................................................................................2.5mg
MnSO4·4H2O .............................................................................2.5mg
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of yeasts, molds, and bacteria found in brew
ing and other industrial fermentation processes.



