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培養基配方
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WL Nutrient Agar (Wallerstein Laboratory Nutrient Agar)
[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]
WL Nutrient Agar
(Wallerstein Laboratory Nutrient Agar)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Glucose ....................................................................................... 50.0g
Agar ............................................................................................ 20.0g
Pancreatic digest of casein............................................................ 5.0g
Yeast extract.................................................................................. 4.0g
KH2PO4....................................................................................... 0.55g
KCl............................................................................................ 0.425g
CaCl2·2H2O .............................................................................. 0.125g
MgSO4·7H2O............................................................................ 0.125g
Bromcresol Green..................................................................... 0.022g
FeCl3 .......................................................................................... 2.5mg
MnSO4·4H2O............................................................................. 2.5mg
Na2CO3 (1% solution)............................................................30.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat with mix
ing and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the detection of bacteria and yeasts from industrial fermenta
tion processes, particularly from beer processing.
