培養基配方

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WL Differential HiVeg Agar

[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓普生物工程有限公司(si) 培養(yang)基配方 //ljxinglong.com

Composition per liter:

Glucose ....................................................................................... 50.0g

Agar ............................................................................................ 20.0g

Plant hydrolysate........................................................................... 5.0g

Yeast extract.................................................................................. 4.0g

KH2PO4....................................................................................... 0.55g

KCl............................................................................................ 0.425g

MgSO4 ...................................................................................... 0.125g

CaCl2......................................................................................... 0.125g

Bromcresol Green ..................................................................... 0.022g

Cycloheximide ...........................................................................4.0mg

FeCl3 ..........................................................................................2.5mg

MnSO4 .......................................................................................2.5mg

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for

mation and inhalation.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat with mix

ing and bring to boiling. Distribute into tubes or flasks. Autoclave for

15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave

in tubes.

Use: For the differential cultivation of bacteria from industrial fermen

tation processes. Growth of yeasts and molds is inhibited. For the

selective isolation and enumeration of bacteria encountered in brewer

ies and industrial fermentations.