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Wickerham Broth with Raffinose

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wickerham Broth with Raffinose

山(shan)東(dong)拓普生(sheng)物工程有(you)限(xian)公司 培(pei)養基配方 //ljxinglong.com

Composition per 100.0mL:

Raffinose..................................................................................... 20.0g
Yeast nitrogen base ................................................................100.0mL
Yeast Nitrogen Base, 10X:
Composition per liter:
Glucose ....................................................................................... 10.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O................................................................................ 0.5g
NaCl.............................................................................................. 0.1g
CaCl2·2H2O .................................................................................. 0.1g
DL-Methionine............................................................................. 0.02g
DL-Tryptophan............................................................................. 0.02g
L-Histidine·HCl ........................................................................... 0.01g
Inositol ....................................................................................... 2.0mg
H3BO3 ........................................................................................ 0.5mg
ZnSO4·7H2O .............................................................................. 0.4mg
MnSO4·4H2O............................................................................. 0.4mg
Thiamine·HCl ............................................................................ 0.4mg
Pyridoxine.................................................................................. 0.4mg
Niacin......................................................................................... 0.4mg
Calcium pantothenate ................................................................ 0.4mg
p-Aminobenzoic acid................................................................. 0.2mg
Riboflavin .................................................................................. 0.2mg
FeCl3 .......................................................................................... 0.2mg
Na2MoO4·4H2O ......................................................................... 0.2mg
KI ............................................................................................... 0.1mg
CuSO4·5H2O............................................................................ 0.04mg
Folic acid ....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
pH 4.5 ± 0.2 at 25°C
Preparation of Yeast Nitrogen Base: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: To 100.0mL of yeast nitrogen base, add
20.0g of raffinose. Mix thoroughly. Filter sterilize. Aseptically distribute
0.5mL into tubes containing 4.5mL of sterile distilled/deionized water.
Use: For the cultivation and differentiation of bacteria based on carbo
hydrate assimilation.
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