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Wickerham Broth

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wickerham Broth

山(shan)東拓普生物工程有限公司(si) 培養(yang)基配方 //ljxinglong.com

Composition per 100.0mL:

KNO3 .......................................................................................... 0.78g
Yeast carbon base...................................................................100.0mL
pH 4.5 ± 0.2 at 25°C
Yeast Carbon Base:
Composition per liter:
Glucose ....................................................................................... 10.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O ................................................................................ 0.5g
NaCl .............................................................................................. 0.1g
CaCl2·2H2O................................................................................... 0.1g
DL-Methionine............................................................................. 0.02g
DL-Tryptophan............................................................................. 0.02g
L-Histidine·HCl ........................................................................... 0.01g
Inositol .......................................................................................2.0mg
H3BO3 ........................................................................................0.5mg
ZnSO4·7H2O ..............................................................................0.4mg
MnSO4·4H2O .............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl3 ..........................................................................................0.2mg
Na2MoO4·4H2O .........................................................................0.2mg
KI ...............................................................................................0.1mg
CuSO4·5H2O ............................................................................0.04mg
Folic acid.....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
Preparation of Yeast Carbon Base: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: To 100.0mL of yeast carbon base, add
0.78g of KNO3 (or peptone). Mix thoroughly. Filter sterilize. Asepti
cally distribute 0.5mL into tubes containing 4.5mL of sterile distilled/
deionized water.
Use: For the cultivation and differentiation of bacteria based on nitrate
assimilation.
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