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WCX Agar

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
WCX Agar

山(shan)東拓普(pu)生(sheng)物(wu)工程有限公(gong)司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 15.0g
CaCl2·2H2O .................................................................................. 1.0g
Cycloheximide solution.........................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Cycloheximide Solution
Composition per 100.0mL:
Cycloheximide........................................................................... 2.5mg
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 100.0mL. Mix thorough
ly. Filter sterilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for
mation and inhalation.
Preparation of Medium: Add components, except cycloheximide
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of myxobacteria.
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