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Wallenstein Medium

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Wallenstein Medium

山東拓普(pu)生(sheng)物工程(cheng)有限公司 培(pei)養基配方 //ljxinglong.com

Composition per 4.225L:

Malachite Green.......................................................................... 0.75g
Egg yolk emulsion ....................................................................3.125L
Glycerol .................................................................................100.0mL
pH 6.75 ± 0.2 at 25°C
Egg Yolk Emulsion:
Composition:
Chicken egg yolks............................................................................ 66
Whole chicken egg............................................................................. 6
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 6 chicken eggs.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation of Mycobacterium species other than Mycobac
terium leprae.
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